| Objective: To explore Multislice CT (MSCT) and MRI in the diagnosis of acute pancreatitis clinical application value. Methods: Clinically suspected acute pancreatitis were retrospectively analyzed clinical, MSCT and MRI data of 66 patients, using MSCT and MRI inspection, the two kinds of imaging results compared with the pathological results come to the conclusion. Methods: 66 patients with clinically suspected for acute pancreatitis, MSCT in the diagnosis of acute pancreatitis is positive 43 cases, accounting for 65.15% (43/66); MSCT scan mainly for pancreas volume increases, the density of the uniform or non-uniform, bleeding area for high density foci, liquefied necrotic for low density foci, enhanced scanning of pancreas in homogeneous or inhomogeneous obvious reinforcement, liquefied necrotic area without reinforcement. MRI in the diagnosis of acute pancreatitis is positive 53 cases, 77.27% (51/66); MRI mainly for pancreas swelling volume, pancreas and peripancreatic fluid has low T1 T2 signal. Results: the pathological diagnosis of acute pancreatitis is positive 52 cases, 78.79% (52/66). The sensitivity and accuracy of the MRI diagnosis of acute pancreatitis (96.15%, 95.45%) was obviously higher than MSCT (75.00%, 74.24%), the difference was statistically significant (χ2=7.790, 9.959, P＜0.05). Specificity of MRI diagnosis of acute pancreatitis (92.86%) than MSCT (71.43%), but there was no statistically significant difference (χ2=0.974, P=0.974). Conclusion: MRI the sensitivity, specificity and accuracy of the diagnosis of acute pancreatitis were higher than MSCT, MSCT examination for acute pancreatitis patients with contraindications, need multiple reexamination, MRI has more advantages.